The winning margin was 0.01 seconds which as of July 2024 is the only time the women's 400 metres has been won by less than a tenth of a second at these championships. Of the six finalists, the winner had the slowest reaction time.
Medallists
edit| Gold | ||
 |
Olesya Zykina |  Russia
|
| Silver | ||
 |
Natalya Nazarova | Russia
|
| Bronze | ||
 |
Shareese Woods |  United States
|
Heats
edit| Heat | Lane | Name | Country | Mark | Q | React |
|---|---|---|---|---|---|---|
| 1 | 6 | Olesya Zykina | Russia |
51.96 | Q | 0.244 |
| 1 | 4 | Moushaumi Robinson | United States |
52.45 | Q | 0.258 |
| 1 | 3 | Makelesi Batimala |  Fiji |
54.02 PB | Q | 0.217 |
| 1 | 1 | Nikolett Listar |  Hungary |
55.37 | 0.237 | |
| 1 | 2 | Martina Xuereb |  Malta |
57.62 NR | 0.282 | |
| 1 | 5 | Moya Thompson |  Jamaica |
DNF | 0.315 | |
| 2 | 5 | Natalya Nazarova | Russia |
52.40 | Q | 0.157 |
| 2 | 3 | Antonina Yefremova |  Ukraine |
52.64 | Q | 0.163 |
| 2 | 6 | Shareese Woods | United States |
52.65 | Q | 0.240 |
| 2 | 4 | Zuzana Hejnová |  Czech Republic |
53.04 | q | 0.146 |
| 2 | 1 | Racheal Nachula |  Zambia |
53.52 NR | q | 0.257 |
| 2 | 2 | Vera Barbosa |  Cape Verde |
57.55 | 0.281 | |
| 3 | 6 | Amantle Montsho |  Botswana |
52.96 PB | Q | 0.290 |
| 3 | 2 | Angela Moroșanu |  Romania |
53.32 SB | Q | 0.249 |
| 3 | 5 | Christy Ekpukhon Ihunaegbo |  Nigeria |
53.34 | Q | 0.187 |
| 3 | 3 | Nataliya Pyhyda | Ukraine |
53.44 | q | 0.187 |
| 3 | 4 | Tsvetelina Kirilova |  Bulgaria |
53.66 | 0.195 | |
| 3 | 1 | Sandrine Thiébaud-Kangni |  Togo |
54.02 SB | 0.209 |
Semifinals
edit| Heat | Lane | Name | Country | Mark | Q | React |
|---|---|---|---|---|---|---|
| 1 | 5 | Olesya Zykina | Russia |
51.75 | Q | 0.315 |
| 1 | 3 | Antonina Yefremova | Ukraine |
51.79 PB | Q | 0.228 |
| 1 | 6 | Moushaumi Robinson | United States |
51.85 PB | Q | 0.248 |
| 1 | 1 | Racheal Nachula | Zambia |
53.30 NR | 0.317 | |
| 1 | 2 | Makelesi Batimala | Fiji |
54.23 | 0.275 | |
| 1 | 4 | Christy Ekpukhon Ihunaegbo | Nigeria |
DNS | ||
| 2 | 6 | Natalya Nazarova | Russia |
51.62 | Q | 0.287 |
| 2 | 3 | Shareese Woods | United States |
51.87 PB | Q | 0.193 |
| 2 | 4 | Angela Moroșanu | Romania |
52.83 SB | Q | 0.273 |
| 2 | 2 | Zuzana Hejnová | Czech Republic |
53.16 | 0.142 | |
| 2 | 5 | Amantle Montsho | Botswana |
53.21 | 0.266 | |
| 2 | 1 | Nataliya Pyhyda | Ukraine |
53.33 |
Final
edit| Heat | Lane | Name | Country | Mark | React |
|---|---|---|---|---|---|
 |
6 | Olesya Zykina | Russia |
51.09 WL | 0.297 |
 |
5 | Natalya Nazarova | Russia |
51.10 SB | 0.247 |
 |
3 | Shareese Woods | United States |
51.41 PB | 0.237 |
| 4 | 4 | Antonina Yefremova | Ukraine |
51.53 PB | 0.147 |
| 5 | 2 | Angela Moroșanu | Romania |
53.07 | 0.269 |
| 6 | 1 | Moushaumi Robinson | United States |
53.10 | 0.257 |