Matysiak is a Polish surname.
Notable people with this surname include:
- Andrzej Matysiak (born 1948),Polish canoer
- Bartłomiej Matysiak (born 1984),Polish cyclist
- Paulina Matysiak (born 1984), Polish politician
- Matysiak family, Polish Matysiakowie is a Polish radio drama, since 1956.